tcba255 MDM2 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using MDM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon using MDM2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using MDM2 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using MDM2 Antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of A549 cells using MDM2 antibody. Blue: DAPI for nuclear staining.
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Product Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Information

ApplicationWB, IHC, IF
Research AreaCancer, Cell cycle, p53 pathway, AKT pathway, Cell Biology, PI3K-Akt pathway, Cell Cycle,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesACTFS;hdm2;HDMX
SwissProtQ00987
Gene ID4193 (human);
Calculated Molecular Weight11-14kDa/24-55kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,nucleolus,nucleoplasm,
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