tcba179 MCL1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using MCL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded mouse kidney using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human prostate using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using MCL1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using MCL1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of A431 cells using MCL1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.

Information
ApplicationWB, IHC, IF
Research AreaCancer, Apoptosis, Cell Biology, Metabolism,
Species ReactivityHuman, Mouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative Namesbcl2-L-3;BCL2L3;EAT;Mcl-1;MCL1-ES;mcl1/EAT;MCL1L;MCL1S;TM
SwissProtQ07820
Gene ID4170 (human);
Calculated Molecular Weight28kDa/37kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Membrane,Mitochondrion,Nucleus,Single-pass membrane protein,nucleoplasm,
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