tcba177 KRT20 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using KRT20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat intestine using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat intestine using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using KRT20 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using KRT20 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using KRT20 antibody. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Information
ApplicationWB, IHC, IF
Research AreaCancer, Tumor biomarkers,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCD20;CK-20;CK20;K20;KRT21
SwissProtP35900
Gene ID54474 (human);
Calculated Molecular Weight48kDa
PurificationAffinity purification
Cellular LocationCytoplasm,
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