tcba139 Bcl-2 Rabbit Polyclonal Antibody

Western blot analysis of extracts of HL-60 cells, using BCL2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Western blot analysis of extracts of various cells, using BCL2 antibody (tcba139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (tcba139) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.Immunohistochemistry of paraffin-embedded human liver cancer using BCL2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using BCL2 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using BCL2 antibody (tcba139) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using BCL2 antibody (tcba139) at dilution of 1:100 (40x lens).Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (tcba139). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (tcba139) at a dilution of 1:1000.Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (tcba139) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Information

ApplicationWB, IHC, IF, IP
Research AreaCancer, Stem cells, Apoptosis, Autophagy pathway, AKT pathway, Neuroscience, Cell Biology, Metabolism, Neurodegeneration, PI3K-Akt pathway,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesBcl-2; PPP1R50; BCL2
SwissProtP10415
Gene ID596 (human);
Calculated Molecular Weight22kDa/26kDa
Observed Molecular Weight26KDa
PurificationAffinity purification
Cellular LocationEndoplasmic reticulum membrane,Mitochondrion outer membrane,Nucleus membrane,Single-pass membrane protein,
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