tcip3047 Recombinant Ebola virus Glycoprotein minus the Transmembrane Region (EBOV rGPΔTM)

Anti-EBOV human mAb KZ52, starting at 50 µg/mL followed by serial dilutions, was incubated with the rVSV pseudotyped with EBOV GP, for one hour prior to adding to Vero cells. Infectivity was determined the next day by assessing luciferase activity. Percent neutralization was calculated based on the control (rVSV pseudotyped with EBOV GP, alone)
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Product Description
Mature, recombinant, His-tagged Ebola

virus Glycoprotein minus the transmembrane domain

(EBOV rGPΔTM) is supplied as purified protein. EBOV

rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC.

Information
ApplicationELISA, WB
Research AreaVirology

Specifications
ConcentrationSupplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 1.591 mg/mL
Recommended DilutionWestern Blot: Quality control testing demonstrates
strong detection of GP null and GP1 under reduced
conditions.

Misc Information
Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceRecombinant glycoprotein provides a means as a control protein for immunoassays and a tool toenhance research.
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