tcip3041 Recombinant Influenza Hemagglutinin (H9 Hong Kong) Full-Length Glycoprotein (rHA GP)

The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms. (A) SDS-PAGE of rHA under reduced condition :1 µg. (B) Western blot detection of rHA at 100 ng, using an anti-HA (H1N1) polyclonal antibody at 0.5 µg/mL and an anti-rabbit IgG-HRP conjugate, followed by TMB membrane substrate.Toxin Functionality: Human promyleocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of Hlg B tag-free and Hlg C at equimolar concentration for 3 hours at 37°C with 5% CO 2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. OD’s were determined in the supernatants at 470/690 nm. Red squares represent the current lot of HIgC tag-free (1509003) and blue square represents the previous HIgC his-tag (Cat # 1403-002, Lot# 1211003). EC 50 was found to be 2.84 nM for current lot and 5.53 nM for HIgC his-tag (Cat # 1403-002, Lot# 1211003).
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Product Description
Recombinant, Influenza Hemagglutinin Full-Length Glycoprotein (rHA GP) from virus strain A/Hong Kong/33982/2009 (H9N2). Recombinant HA is supplied as an affinity purified protein. rHA is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC

Information
ApplicationELISA, WB
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied at a concentration of 0.33 mg/mL (by BCA) in Tris buffered saline plus 0.01% non-ionic detergent. ; The theoretical molecular weight of the protein is ~63 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, mig
Recommended DilutionWestern Blot: Quality control testing demonstrates detection of GP null under reduced conditions when using anti-H9 influenza antiserum.

Hemagglutination with Turkey Red Blood Cells: HA Titer 1:32
768
Purity / GradeColumn chromatography (FPLC); Purity: Residual baculovirus GP64 co-purifies with the affinity purified rHA GP and was determined to be less the 10% of the total protein.

Misc Information
Storage Instruction2-3 weeks at -20°C, long term It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles
RelevanceRecombinant hemagglutinin glycoprotein provides a control protein for immunoassays and a tool to enhance Orthomyxovirus research.
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