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Protein & Peptides
Ebola virus-like particles (EBOV VLP)
tcip3021
Ebola virus-like particles (EBOV VLP)
Quality control testing shows two prominent bands: E3E2 at ~50 kDa and cleaved E2 at ~40 kDa.(A) SDS-PAGE and stain demonstrating 1 µg and 5 µg (lanes 1-2) of EEEV E3E2/E2 protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers. (B) Western blot detection of EEEV E3E2/E2 protein at 50 ng, 100 ng, and 200 ng (lanes 1-3) using Taiclone anti-EEEV E2 rabbit polyclonal antibody (Taiclone product No.tcia164) at 0.5 µg/mL and anti-rabbit IgGHRP conjugate, followed by TMB substrate.
Western Blot was used to confirm the presence of EBOV-specific GP, NP and VP40 in the VLP. VLP sample was heat-denatured under reducing condition and loaded at 1 µg/lane. (A) GP was detected as a broadly-diffused band 60- 110 kDa using 10 ng/mL of anti-EBOV GP rabbit Pab (Taiclone #tcia135) (B) NP was detected as a single sharp band at ~90 kDa using 100 ng/mL of anti-EBOV NP rabbit Pab (Taiclone#tcia134) (C) VP40 was detected as a single sharp band at ~40 kDa using 50 ng/mL of anti-EBOV VP40 rabbit Pab (Taiclone#tcia133)
Plate was coated with EEEV E3E2/E2 protein starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using Rabbit Anti-EEEV E2 pAb at 1 µg/mL. OD 650 is reported.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Product Description
Virus-like Particles (VLP’s) expressing recombinant Ebola virus (EBOV)
glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). These VLP’s are produced in Sf9 insect cells through infection with a recombinant baculovirus.
Information
Application
WB, ELISA
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in PBS (supplemented with arginine and glutamic acid) at concentration of 1.87 mg/mL.(Lot dependent)
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
Since these VLP's mimic Ebola virus but do not contain genetic material, thus are not infectious and make an ideal candidate as a vaccine and also as a tool to enhance filovirus research.
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