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Protein & Peptides
S. aureus Recombinant LukF-PV (tag-free)
tcip3020
S. aureus Recombinant LukF-PV (tag-free)
Western Blot was used to confirm the presence of EBOV-specific GP, NP and VP40 in the VLP. VLP sample was heat-denatured under reducing condition and loaded at 1 µg/lane. (A) GP was detected as a broadly-diffused band 60110 kDa using 10 ng/mL of anti-EBOV GP rabbit Pab (Taiclone's cat# tcia135) (B) NP was detected as a single sharp band at ~90 kDa using 100 ng/mL of anti-EBOV NP rabbit Pab (Taiclone's cat# tcia134) (C) VP40 was detected as a single sharp band at ~40 kDa using 50 ng/mL of anti-EBOV VP40 rabbit Pab (Taiclone' cat# tcia133)
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AVAILABLE SIZES
100µg
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0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Purified, recombinant Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV). ;
The theoretical molecular weight of the protein is 34,580 Daltons.
Information
Application
ELISA, WB, Cytotoxicity
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
Supplied in sodium phosphate buffer containing sodium chloride and 5% glycerol, at a concentration of 1.624 mg/mL.
Recommended Dilution
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution
internal QC demonstrates detection of 100 ng of LukF-PV protein when detected with antiPVL LukF polyclonal antibody (cat# tcia158) in Western blotting.
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV (cat# tcip3019) in a concentration range of 1-100 nM.
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
This protein may be used in functional PVL toxicity assays in combination with LukS-PV, or as a control protein in ELISA assays or Western blotting when detecting LukF in PVL (+) strains of S. aureus.
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