tcip3008 Recombinant BDBV GPDTM

The theoretical molecular weight of the protein is ~60 kDa including the His-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDSPAGE gel is slowed resulting in broad, diffuse bandsrepresenting differing glycosylationforms..(A) SDS-PAGE and stain demonstrating 5 g and 1 g (lane 1, 2 respectively) of MARV-Angola rGPΔTM His-tag protein underdenaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers. (B) Western blot detection of MARVAngola rGPΔTM at 50 ng, 100 ng and 500 ng (lanes 1-3). MARVAngola rGPΔTM was detected using Taiclone's polyclonal antibody (catalog # tcia147) at 0.5u g/mL and anti-rabbit IgG-HRP conjugate, followed by substrate. Plate was coated with MARV-Angola rGPΔTM starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with antiIgGHRP conjugate and TMB substrate.OD650 is reported. Background of MARV-Angola rGPΔTM coated plate without positive control serum was 0.053 OD 650 .
Order Now

AVAILABLE SIZES

 $0.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description
Mature, recombinant, His-tagged Bundibugyo glycoprotein minus the transmembrane domain (BDBV rGPΔTM) is supplied as purified protein. BDBV rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by column chromatography;The theoretical molecular weight of the protein is ~68 kDa including the His-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDSPAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.

Information
ApplicationELISA, WB
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied in PBS (supplemented with 10% glycerol, arginine and glutamic acid) at a concentration of 1.59 mg/mL (Lot Dependent)
Purity / GradeColumn chromatography

Misc Information
Storage Instruction2-3 weeks at -20°C, -80°C long term
RelevanceRecombinant BDBV glycoprotein provides a means as a control protein for immunoassays and a tool to enhance Filovirus research.
Get valuable resources and offers directly to your email.
COMPANY About Taiclone Shipping Terms Taiclone Guarantee
RESOURCES Life Science Glossary
PRODUCTS Primary AntibodySecondary AntibodyProtein & PeptidesBioChemicalsAssay & Elisa KitsLoading ControlOther ReagentsSerumsCell Line
SUPPORT Contact Us Email Us Terms of Service Privacy Policy
Copyright 2016-2023 Taiclone Biotech Corp. All Rights Reserved.