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Protein & Peptides
Recombinant EBOV GPDTM (mammalian)
tcip3002
Recombinant EBOV GPDTM (mammalian)
The theoretical molecular weight of the protein is ~70.5 kDa including the HA-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDSPAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms. (A) SDS-PAGE and stain demonstrating 5 ug, (lane 1, 2 respectively) of EBOV rGPΔTM HA-Tag protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers. (B) Western blot detection of EBOV rGPΔTM at 500 ng, 100 ng, and 50 ng (lanes 1-3). EBOV rGPΔTM was detected using IBT’s monoclonal antibody 4F3 at 0.5 ug/mL and anti-mouse IgG-HRP conjugate, followed by TMB substrate.
Plate was coated with EBOV rGPΔTM starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMBsubstrate. OD 650 is reported.
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AVAILABLE SIZES
100µg
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0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
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Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Mature, recombinant, HA-tagged ebolavirus glycoprotein minus the transmembrane domain (EBOV rGPΔTM) is supplied as purified protein. EBOV rGPΔTM is produced in mammalian cells and is purified by FPLC.
Information
Application
ELISA, WB
Research Area
Virology
Specifications
Form
Frozen Liquid
Concentration
supplied in PBS at a concentration of 3.831 mg/mL
Recommended Dilution
Quality control testing demonstrates
strong detection of GP null under reduced conditions
down to 50 ng when using Taiclone's monoclonal antibody 4F3 (cat# tcia113) at 0.5 g/mL
Purity / Grade
Column chromatography
Misc Information
Storage Instruction
2-3 weeks at -20°C, -80°C long term
Relevance
Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.
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