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Primary Antibody
Mouse anti-EBOV VP40 Monoclonal Antibody (3G5)
tcia110
Mouse anti-EBOV VP40 Monoclonal Antibody (3G5)
Western blots were detected under reduced conditions with anti-EBOV VP40 mAb 3G5 at 10 ng/mL and visualized using an anti-mouse IgG-HRP conjugate and TMB membrane substrate. rVP40 was loaded at 100 ng per lane. SUDV rVP40 and EBOV rVP40 are visualized in lanes 1 and 2, respectively. VLP was loaded at 1 µg per lane. VP40 is detected in SUDV VLP (lane 4) and EBOV VLP (lane 5).
rVP40 proteins and VLPs were diluted to 1 and 10 µg/mL, respectively in PBS for plate coating. Anti-EBOV VP40 antibody 3G5 was serially diluted semi-log dilutions from 10 µg/mL and incubated on the coated plates. Washed plates were detected with anti-mouse IgG-HRP conjugate and TMB substrate. OD650 is reported above
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AVAILABLE SIZES
100µg
$
531.18
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Product Description
Protein G purified mouse monoclonal antibody reactive to EBOV VP40. The antibody detects VP40 in virus-like particles (VLP) and recombinant protein.
Information
Application
ELISA, WB
Research Area
Virology
Cross reactivity
Cross reactivity was observed againstSudan Virus (SUDV) VP40 in VLP and SUDV rVP40. No cross reactivity was observed with Marburg Virus (MARV) VP40 in VLP or MARV rVP40.
Host Species
Mouse
Immunogen / Amino acids
EBOV VP40 protein
Conjugation
Unconjugated
Clonality
monoclonal
Clones
monoclonal
Isotype
IgG1
Specifications
Form
Frozen Liquid,Protein A/G Purified
Concentration
Supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 0.718 mg/mL. No preservative is added.
Recommended Dilution
ELISA: Assay-dependent dilution
Western Blot: Assay-dependent dilution. Internal QC demonstrates good detection when using 3G5 mAb at a use dilution of 10 ng/mL.
Misc Information
Storage Instruction
2-3 weeks +4°C, -20°C long term. Avoid multiple freeze thaws.
Relevance
The antibody can be used for detection of EBOV VP40.
Purification
Protein A/G
related data
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