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Primary Antibody
Biotinylated Anti-Mouse IgG2b rabbit monoclonal antibody [RM108]
tcra313b
Biotinylated Anti-Mouse IgG2b rabbit monoclonal antibody [RM108]
Western blot of nonreduced(-) and reduced(+)mouse IgG2b (20ng/lane), using 0.2ug/mL ofRevMAb clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much strongerthan the reduced γ2b form (~50 kDa).
ELISA of mouse immunoglobulins shows RM108reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosph-atase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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AVAILABLE SIZES
50ug
$
319.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Biotinylated Rabbit monoclonal to Mouse IgG2b;This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Information
Application
WB (stronger on nonreduced), IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG2b
Conjugation
Biotin
Clonality
Monoclonal
Clones
RM108
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005 ug/mL – 0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P01867
Gene ID
16016
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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