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Primary Antibody
Biotinylated Anti-Mouse IgG2a rabbit monoclonal antibody [RM219]
tcra311b
Biotinylated Anti-Mouse IgG2a rabbit monoclonal antibody [RM219]
ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was usedas the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondaryantibody.
A titer ELISA of mouse IgG2a. The plate wascoated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primaryantibody. An alkaline phosphatase conjugatedanti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+)mouse IgG2a, using 0.5ug/mL of RevMAb cloneRM219. This antibody reacts to nonreducedIgG2a (~150 kDa).
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AVAILABLE SIZES
50ug
$
329.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Biotinylated Rabbit monoclonal to Mouse IgG2a ;This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Information
Application
WB (nonreduced only), IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG2a
Conjugation
Biotin
Clonality
Monoclonal
Clones
RM219
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005 ug/mL – 0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.5 ug/mL-2 ug/mL.
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P01863
Gene ID
380793
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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