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Primary Antibody
Biotinylated Anti-Mouse IgG rabbit monoclonal antibody [RM104]
tcra101b
Biotinylated Anti-Mouse IgG rabbit monoclonal antibody [RM104]
Western blot of nonreduced(-) and reduced(+)mouse immunoglobulins (20ng/lane), using 0.2ug/mL of RevMAb clone RM104. This antibodyreacts to nonreduced mouse IgG1, IgG2a, IgG2b,and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA of mouse immunoglobulins shows RM104reacts to the Fc region of mouse IgG1, IgG2a,IgG2b, and IgG3; no cross reactivity with IgM,IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG. The plate was coatedwith different amounts of mouse IgG. A serial dilutionof RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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AVAILABLE SIZES
50ug
$
319.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Biotinylated Rabbit monoclonal to Mouse IgG;This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
Information
Application
WB (nonreduced), IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG
Conjugation
Biotin
Clonality
Monoclonal
Clones
RM104
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005 ug/mL-0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P01868
Gene ID
16017
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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