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Primary Antibody
Biotinylated Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]
tcra106b
Biotinylated Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]
Western blot of nonreduced(-) and reduced(+)mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mLof RevMAb clone RM103. This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
ELISA of mouse immunoglobulins shows RM103reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL ofRM103 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.
A titer ELISA of mouse IgG1κ. The plate was coatedwith different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.
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AVAILABLE SIZES
50ug
$
329.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Biotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain;This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
Information
Application
WB (nonreduced), IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG
Conjugation
Biotin
Clonality
Monoclonal
Clones
RM103
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005ug/mL-0.2ug/mL
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Western Blot (WB): 0.1ug/mL-0.5ug/mL.
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P01837
Gene ID
16071
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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