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Primary Antibody
Anti-Mouse IgG1 rabbit monoclonal antibody [RM106]
tcra102
Anti-Mouse IgG1 rabbit monoclonal antibody [RM106]
Western blot of nonreduced(-) and reduced(+)mouse IgG1 (20ng/lane), using 0.2ug/mL of RevMAb clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than thereduced γ1 form (~50 kDa).
ELISA of mouse immunoglobulins shows RM106reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1. The plate was coatedwith different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbit IgG asthe secondary antibody.
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AVAILABLE SIZES
100ug
$
279.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Rabbit monoclonal to Mouse IgG1;This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.
Information
Application
WB, IP, ICC, IHC, FC, ELISA
Species Reactivity
Mouse
Host Species
Rabbit
Immunogen / Amino acids
Mouse IgG
Conjugation
Unconjugated
Clonality
Monoclonal
Clones
RM106
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Concentration
1 mg/mL
Recommended Dilution
ELISA: 0.005-0.2ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)
Western Blot: 0.1-0.5ug/ml
Misc Information
Storage Instruction
store at -20°C ; avoid repeated thawing/freezing
SwissProt
P01868
Gene ID
16017
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Notes
Sold under RevMab BioSciences Labelled.
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