Product Description
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Information
ApplicationPep-ELISA, WB, IHC
Research Areamitochondrial precusor
Species ReactivityHuman, Mouse, Rat, Dog, Pig, Cow
Host SpeciesGoat
Immunogen / Amino acidsDFTEEICRRVKDLD
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 37kDa band observed in lysates of Human Lymph Nodes
and of cell line DAUDI
and 38kDa in Human
Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesIDH3A; isocitrate dehydrogenase 3 (NAD+) alpha; H-IDH alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase subunit alpha
Reference Sequence No.NP_005521.1
Calculated Molecular Weight39.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Positive Controltcva10995p