Product Description
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Information
ApplicationPep-ELISA, WB, IHC
Research Areaprotein sorting
VPS
Species ReactivityHuman, Mouse, Rat, Cow
Host SpeciesGoat
Immunogen / Amino acidsC-SPESEGPIYEGLIL
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 85-90kDa band observed in Human Brain (Cerebellum)
Heart and Rat Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). Recommended concentration: 1-3µg/ml. Preliminary testing was unsuccessful on Mouse Brain for this particular batch. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesVPS35; MEM3; FLJ10752; FLJ13588; FLJ20388; DKFZp434E1211; DKFZp434P1672; vacuolar protein sorting 35 (yeast); maternal-embryonic 3; vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting 35
Reference Sequence No.NP_060676.2
Calculated Molecular Weight91.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
NotesNote there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Positive Controltcva06268p