Product Description
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Information
ApplicationPep-ELISA, WB, IF
Research Areaubiquitin-conjugating enzyme
Species ReactivityHuman, Mouse, Rat, Pig, Cow
Host SpeciesGoat
Immunogen / Amino acidsC-EFTKKYGEKRPVD
ConjugationUnconjugated
Specifications
FormLiquid
Storage BufferTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Concentration0.5 mg/ml in 200 µl
Recommended DilutionWestern Blot: Approx 18kDa band observed in lysates of cell lines HeLa HEK293
HepG2 and Jurkat and also in lysates of Human
Mouse and Rat Heart
Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3µg/ml.
Peptide ELISA: antibody detection limit dilution 1:128000.
Misc Information
Storage InstructionAliquot store at -20C. Avoid freeze / thaw cycles.
Alternative NamesUBE2L3; ubiquitin-conjugating enzyme E2L 3; UBCH7; E2-F1; L-UBC; UbcM4; ubiquitin carrier protein; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase
Reference Sequence No.NP_003338.1
Calculated Molecular Weight17.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
DS Gene Ontology Termsprotein modification; ubiquitin--protein ligase; ubiquitin conjugating enzyme; ubiquitin-dependent protein degradation
Positive Controltcva05770p