tcue1879 ELISA Kit for Reverse Triiodothyronine (rT3)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Reverse Triiodothyronine (rT3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Reverse Triiodothyronine (rT3) and unlabeled Reverse Triiodothyronine (rT3) (Standards or samples) with the pre-coated antibody specific to Reverse Triiodothyronine (rT3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Reverse Triiodothyronine (rT3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Reverse Triiodothyronine (rT3) in the sample.