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Assay & Elisa Kits
ELISA Kit for Immunoglobulin A (IgA)
tcue1645
ELISA Kit for Immunoglobulin A (IgA)
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AVAILABLE SIZES
96T
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
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Heat mediated
Enzymatic
Other
None
Positive Control
Negative Control
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1:
Incubation Time *
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Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Information
Research Area
Infection immunity
Immune molecule
Hematology
Pulmonology
Species Reactivity
Rattus norvegicus (Rat)
Sample Type
serum, plasma and other biological fluids
Specifications
Tested Application
ELISA
Sensitivity
The minimum detectable dose of this kit is typically less than 22.85pg/mL
Detection Range
46.88-3000pg/mL
Assay Time
3h
Detection Method
Enzyme-linked immunosorbent assay for Antigen Detection.
Misc Information
SwissProt
P20760
related data
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