tcue359 ELISA Kit for Pro-Gastrin Releasing Peptide (ProGRP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pro-Gastrin Releasing Peptide (ProGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pro-Gastrin Releasing Peptide (ProGRP) and unlabeled Pro-Gastrin Releasing Peptide (ProGRP) (Standards or samples) with the pre-coated antibody specific to Pro-Gastrin Releasing Peptide (ProGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample.