tcsc3640 4-Azido-L-phenylalanine

4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments.

In Vitro: In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions^[1].