tcsc3375 PF-573228

PF-573228 is a potent and selective FAK inhibitor with IC₅₀ of 4 nM for purified recombinant catalytic fragment of FAK.

IC50 & Target: IC50: 4 nM (FAK)^[1]

In Vitro: PF-573228 inhibits purified recombinant catalytic fragment of FAK with an IC₅₀ of 4 nM. In cultured cells, PF-573228 inhibits FAK phosphorylation on Tyr₃₉₇ with an IC₅₀ of 30-100 nM. Treatment of cells with concentrations of PF-573228 that significantly decreased FAK Tyr₃₉₇ phosphorylation fails to inhibit cell growth or induce apoptosis. In contrast, treatment with PF-573228 inhibits both chemotactic and haptotactic migration concomitant with the inhibition of focal adhesion turnover^[1].