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BioChemicals
RITA
tcsc1403
RITA
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5mg
10mg
50mg
$
223.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Product Description
RITA is an inhibitor of
p53-HDM-2 interaction
, binds to p53dN, with a
K
d
of 1.5 nM, and also induces
DNA-DNA cross-links
.
IC50 & Target: Kd: 1.5 nM (p53dN)
[1]
DNA Crosslinker
[2]
In Vitro:
RITA inhibits p53-HDM-2 interaction, binding to p53dN, with a K
d
of 1.5 nM. RITA (10 μM) blocks complex formation between p53 and HDM-2 in HCT116 cells and HDFs and in NHF-ERMyc cells irrespective of c-Myc expression. RITA (0.5 μM) reduces the viability of tumor cells in a wild-type p53-dependent manner. Moreover, RITA (0.1 μM) induces p53-dependent apoptosis. RITA induces p53 but does not via DNA damage-signaling pathway
[1]
. RITA (NSC 652287) induces DNA-DNA cross-links. RITA induces G2-M cell cycle arrest at 10 nM and causes apoptosis at 100 nM. RITA (100 nM) also elevates p53 and causes dose-dependent effects on p
21WAF1
protein levels
[2]
. RITA inhibits the growth of HeLa and CaSki cells, with IC
50
s of 1 and 10 μM. In addition, RITA (1 μM) stabilizes p53 by inhibiting p53/E6AP interaction
[3]
.
In Vivo:
RITA (0.1, 1 or 10 mg/kg, i.p.) shows potent antitumor activity in SCID mice bearing HCT116 and HCT116 TP53
−/−
xenografts
[1]
. RITA (10 mg/kg, i.p.) also suppresses the growth of HeLa cells in SCID mice
[3]
.
Information
CAS No
213261-59-7
Formula
C
14
H
12
O
3
S
2
Clinical Information
clinicalinformation
Pathway
Cell Cycle/DNA Damage
Autophagy
Apoptosis
Target
DNA Alkylator/Crosslinker
Autophagy
MDM-2/p53
Specifications
Purity / Grade
>98%
Solubility
DMSO 205.0 mg/mL (701.2 mM)
Water: Insoluble
Smiles
smiles
Misc Information
Storage Instruction
Powder-20°C for 3 years
In solvent -80°C for 12 months
Alternative Names
NSC 652287
Observed Molecular Weight
292.37
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