tcsc0926 CHAPS

CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins.

Target: CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons.CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.

In Vitro::CHAPS (0.5 %) is able to stabilize complexes between DNA and DNA-binding factors such as AP-1, SPI, GATA-1 and α-regulated factor ISGF3, and retains their biochemical activity[1].

CHAPS can prevent dissociation of mononucleosomes diluted to sub-nanomolar concentrations[1].