tcsc0031562 5α-Pregnane-3β,6α-diol-20-one

5α-Pregnane-3β,6α-diol-20-one is a mitogenic metabolite of progesterone, and it can be produced in starved androgen-responsive prostate cancer cells.

In Vitro: Progesterone is converted to 5α-pregnane-3β,6α-diol-20-one in human fibroblasts^[1]. Pregnenolone and progesterone are avidly metabolized to 5α-pregnan-3β,6α-diol-20-one in the C4.2 cell line, with nearly complete conversions at 24 h and 18 h, respectively. Preferential 5α-pregnan-3β,6α-diol-20-one formation from early steroid precursors is independent of CYP17A1 but rather relies on 3β-HSD activity^[2].