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Assay & Elisa Kits
Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
tcee3469
Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
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AVAILABLE SIZES
96T
$
609.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
This ELISA kit applies to the in vitro quantitative determination of Mouse S100A9 concentrations in serum, plasma and other biological fluids.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100A9. You can calculate the concentration of Mouse S100A9 in the samples by comparing the OD of the samples to the standard curve.
Information
Research Area
Signal transduction, Immunology, Cancer
Species Reactivity
Mouse
Sample Type
Serum, plasma and other biological fluids
Assay Type
Sandwich
Specifications
Tested Application
ELISA
Sensitivity
0.38 ng/mL
Detection Range
0.63—40 ng/mL
Assay Time
4.5h
Detection Method
Colormetric
Misc Information
Storage Instruction
An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the detailed information on the manual once the kit is received.
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