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Primary Antibody
Anti TEAD1 Antibody
tcsa22975
Anti TEAD1 Antibody
Western blot analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody at dilution of 1:100 (40x lens).
Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Information
Application
WB, IHC, IF, ChIP
Species Reactivity
Human, Mouse
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human TEAD1
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
CHIP 1:20 - 1:50
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
TEAD1 antibodyTranscriptional enhancer factor TEF-1 antibodyNTEF-1 antibodyProtein GT-IIC antibodyTEA domain family member 1 antibodyTEAD-1 antibodyTranscription factor 13 antibodyTCF-13 antibodyTEAD1 antibodyT
SwissProt
P28347
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