tcsa21749 Anti RPN1 Antibody

Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody at dilution of 1:100 (20x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody at dilution of 1:100 (40x lens).
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Product Description

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Information

ApplicationWB, IHC
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human RPN1
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications

FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200

Misc Information

Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesRPN1 antibody
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
Ribophorin I antibody
RPN-I antibody
Ribophorin-1 an
SwissProtP04843
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