tcsa21294 Anti MTOR Antibody

Western blot analysis of extracts of various cell lines, using MTOR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat ovary using MTOR antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using MTOR antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using MTOR antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using MTOR antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using MTOR antibody at dilution of 1:150 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using MTOR antibody at dilution of 1:150 (40x lens).Immunofluorescence analysis of HeLa cells using MTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using MTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human MTOR
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesMTOR antibody
Serine/threonine-protein kinase mTOR antibody
MTOR antibody
FRAP antibody
FRAP1 antibody
FRAP2 antibody
RAFT1 antibody
RAPT1 antibody
SwissProtP42345
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