tcsa20808 Anti SMAD9 Antibody

Western blot analysis of extracts of various cell lines, using SMAD9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry of paraffin-embedded human liver injury using SMAD9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of A549 cells using SMAD9 antibody. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human SMAD9
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesSMAD9 antibody
Mothers against decapentaplegic homolog 9 antibody
MAD homolog 9 antibody
Mothers against DPP homolog 9 antibody
Madh6 antibody
SMAD family member 9 antibody
SMAD 9 antibody
Smad9 antibody
SMAD9 antibody
SwissProtO15198
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