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Primary Antibody
Anti Phospho BCL2 S70 Antibody
tcsa20526
Anti Phospho BCL2 S70 Antibody
Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
CELLS
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Product Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Information
Application
WB, IF
Species Reactivity
Human
Host Species
Rabbit
Immunogen / Amino acids
A phospho specific peptide corresponding to residues surrounding S70 of human Bcl2
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
BCL2 antibodyApoptosis regulator Bcl-2 antibodyBCL2 antibody
SwissProt
P10415
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