tcsa20473 Anti MAP1LC3B Antibody

Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of pig liver, using MAP1LC3B antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat heart using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
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Product Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat, Pig
Host SpeciesRabbit
Immunogen / Amino acidsA synthetic peptide of human MAP1LC3B
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesMAP1LC3B antibody
Microtubule-associated proteins 1A/1B light chain 3B antibody
Autophagy-related protein LC3 B antibody
Autophagy-related ubiquitin-like modifier LC3 B antibody
MAP1 light chain 3-like protein 2 antibody
MAP1A/MAP1B li
SwissProtQ9GZQ8
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