tcsa20326 Anti EFTUD2 Antibody

Western blot analysis of extracts of various cell lines, using EFTUD2 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using EFTUD2 antibody.Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody. Western blot was performed from the immunoprecipitate using EFTUD2 antibody at a dilition of 1:1000.
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Product Description
This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene.

Information
ApplicationWB, IHC, IF, IP
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human EFTUD2
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesEFTUD2 antibody
116 kDa U5 small nuclear ribonucleoprotein component antibody
Elongation factor Tu GTP-binding domain-containing protein 2 antibody
SNU114 homolog antibody
hSNU114 antibody
U5 snRNP-specific protein antibody
116 kDa
SwissProtQ15029
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