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Primary Antibody
Anti PNKP Antibody
tcsa20012
Anti PNKP Antibody
Western blot analysis of extracts of various cell lines, using PNKP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using PNKP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Heat mediated
Enzymatic
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Product Description
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human PNKP
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
PNKP antibodyBifunctional polynucleotide phosphatase/kinase antibodyDNA 5'-kinase/3'-phosphatase antibodyPolynucleotide kinase-3'-phosphatase antibodyPNKP antibody
SwissProt
Q96T60
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