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Primary Antibody
Anti SPN Antibody
tcsa19753
Anti SPN Antibody
Western blot analysis of extracts of THP-1 cells, using SPN antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SPN antibody at dilution of 1:100 (40x lens).
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
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Product Description
The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response.
Information
Application
WB, IHC
Species Reactivity
Human, Mouse
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human SPN
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
SPN antibodyLeukosialin antibodyGPL115 antibodyGalactoglycoprotein antibodyGALGP antibodyLeukocyte sialoglycoprotein antibodySialophorin antibodyCD antigen CD43 antibodySPN antibodyCD43 antibody
SwissProt
P16150
related data
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