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Primary Antibody
Anti CHAF1B Antibody
tcsa19421
Anti CHAF1B Antibody
Western blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human CHAF1B
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
CHAF1B antibodyChromatin assembly factor 1 subunit B antibodyCAF-1 subunit B antibodyChromatin assembly factor I p60 subunit antibodyCAF-I 60 kDa subunit antibodyCAF-I p60 antibodyM-phase phosphoprotein 7 antibodyCHAF1B
SwissProt
Q13112
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