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Primary Antibody
Anti FKBP4 Antibody
tcsa19158
Anti FKBP4 Antibody
Western blot analysis of extracts of various cell lines, using FKBP4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver injury using FKBP4 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FKBP4 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using FKBP4 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using FKBP4 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using FKBP4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using FKBP4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using FKBP4 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using FKBP4 antibody. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug FKBP4 antibody. Western blot was performed from the immunoprecipitate using FKBP4 antibody at a dilition of 1:1000.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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Product Description
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene.
Information
Application
WB, IHC, IF, IP
Species Reactivity
Human, Mouse
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human FKBP4
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
FKBP4 antibodyPeptidyl-prolyl cis-trans isomerase FKBP4 antibodyPPIase FKBP4 antibodyFKBP4 antibodyFKBP52 antibody
SwissProt
Q02790
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