tcsa19035 Anti PRPF3 Antibody

Western blot analysis of extracts of various cell lines, using PRPF3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using PRPF3 antibody. Blue: DAPI for nuclear staining.
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Product Description
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human PRPF3
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesPRPF3 antibody
U4/U6 small nuclear ribonucleoprotein Prp3 antibody
Pre-mRNA-splicing factor 3 antibody
hPrp3 antibody
U4/U6 snRNP 90 kDa protein antibody
PRPF3 antibody
HPRP3 antibody
PRP3 antibody
SwissProtO43395
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