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Primary Antibody
Anti RPS6KA3 Antibody
tcsa18990
Anti RPS6KA3 Antibody
Western blot analysis of extracts of mouse lung, using RPS6KA3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
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Heat mediated
Enzymatic
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Product Description
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human RPS6KA3
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
RPS6KA3 antibodyRibosomal protein S6 kinase alpha-3 antibodyS6K-alpha-3 antibodyRPS6KA3 antibodyISPK1 antibodyMAPKAPK1B antibodyRSK2 antibody
SwissProt
P51812
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