tcsa18856 Anti NONO Antibody

Western blot analysis of extracts of various cell lines, using Nono antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry of paraffin-embedded rat liver using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophagus using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using Nono Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using Nono antibody.
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Product Description
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human Nono
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesNONO antibody
Non-POU domain-containing octamer-binding protein antibody
NonO protein antibody
54 kDa nuclear RNA- and DNA-binding protein antibody
55 kDa nuclear protein antibody
DNA-binding p52/p100 complex antibody
52 kDa subuni
SwissProtQ15233
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