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Primary Antibody
Anti HSPB8 Antibody
tcsa17095
Anti HSPB8 Antibody
Western blot analysis of extracts of various cell lines, using HSPB8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human normal colon using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using HSPB8 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB8 antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human HSPB8
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
HSPB8 antibodyHeat shock protein beta-8 antibodyHspB8 antibodyAlpha-crystallin C chain antibodyE2-induced gene 1 protein antibodyProtein kinase H11 antibodySmall stress protein-like protein HSP22 antibodyHSPB8 antibody
SwissProt
Q9UJY1
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