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Primary Antibody
Anti GGA2 Antibody
tcsa16885
Anti GGA2 Antibody
Western blot analysis of extracts of various cell lines, using GGA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GGA2 antibody. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human GGA2
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
GGA2 antibodyADP-ribosylation factor-binding protein GGA2 antibodyGamma-adaptin-related protein 2 antibodyGolgi-localized antibodygamma ear-containing antibodyARF-binding protein 2 antibodyVHS domain and ear domain of gamma-a
SwissProt
Q9UJY4
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