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Primary Antibody
Anti EDF1 Antibody
tcsa16683
Anti EDF1 Antibody
Western blot analysis of extracts of various cell lines, using EDF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using EDF1 antibody at dilution of 1:100 (40x lens).
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
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Formaldehyde
Methanol
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Heat mediated
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Product Description
This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants.
Information
Application
WB, IHC
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human EDF1
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
EDF1 antibodyEndothelial differentiation-related factor 1 antibodyEDF-1 antibodyMultiprotein-bridging factor 1 antibodyMBF1 antibodyEDF1 antibody
SwissProt
O60869
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