tcsa16628 Anti DKC1 Antibody

Western blot analysis of extracts of various cell lines, using DKC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded human colon using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophagus using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse pancreas using DKC1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using DKC1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using DKC1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human cancer using DKC1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using DKC1 Antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using DKC1 Antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of U2OS cells using DKC1 antibody. Blue: DAPI for nuclear staining.
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Product Description
This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants.

Information
ApplicationWB, IHC, IF
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant protein of human DKC1
ConjugationUnconjugated
ClonalityPolyclonal
IsotypeIgG

Specifications
FormLiquid
Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20C. Avoid freeze / thaw cycles.
Alternative NamesDKC1 antibody
H/ACA ribonucleoprotein complex subunit 4 antibody
DKC1 antibody
NOLA4 antibody
SwissProtO60832
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