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Primary Antibody
Anti AHR Antibody
tcsa16076
Anti AHR Antibody
Western blot analysis of extracts of various cell lines, using AHR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using AHR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using AHR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AHR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AHR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using AHR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AHR antibody at dilution of 1:100 (40x lens).
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AVAILABLE SIZES
100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
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Product Description
The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
Information
Application
WB, IHC
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant protein of human AHR
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:200 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
AHR antibodyAryl hydrocarbon receptor antibodyAh receptor antibodyAhR antibodyClass E basic helix-loop-helix protein 76 antibodybHLHe76 antibodyAHR antibodyBHLHE76 antibody
SwissProt
P35869
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