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Primary Antibody
Anti HDAC4 Antibody
tcsa12796
Anti HDAC4 Antibody
Western blot analysis of extracts of various cell lines, using HDAC4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using HDAC4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using HDAC4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HDAC4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HDAC4 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HDAC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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100µl
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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CELLS
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Product Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Information
Application
WB, IHC, IF
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
A synthetic peptide of human HDAC4
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20C. Avoid freeze / thaw cycles.
Alternative Names
HDAC4 antibodyHistone deacetylase 4 antibodyHD4 antibodyHDAC4 antibodyKIAA0288 antibody
SwissProt
P56524
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