Product Description
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer.
The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Information
Species ReactivityHuman
Host SpeciesHEK293T
Specifications
FormLiquid
Storage Buffer25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Concentration>50 ug/mL as determined by microplate BCA method
Protein LengthRecombinant protein was produced with GoldORF clone, [GH51725V]. Click on the GoldORF clone link to view cDNA and protein sequences.
Purity / Grade> 80% as determined by SDS-PAGE and Coomassie blue staining
Misc Information
Storage InstructionStable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquotand store at -20°C or below.
Avoid repeated freeze-thaw cycles.
Alternative NamesALK,CD246; NBLST3
Calculated Molecular Weight176.3 kDa
TagsC-Myc/DDK
NotesmRNA Refseq :NM_004304
Protein Refseq :NP_004295
FunctionACTIVITY:
ALK activity verified in a biochemical assay: ALK (anaplastic lymphoma receptor tyrosine kinase) (PH34203M5) activity was measured in a homogeneous time resolved fluorescent (HTRF®) assay. ALK is an orphan receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. Varying concentrations of ALK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader.
The time resolved fluorescent signal is expressed as “delta R” or “R” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.