tcba021983 Mouse anti mCherry-Tag Monoclonal Ab

Western blot analysis of over-expressed mCherry-tagged protein in 293T cells using Mouse anti mCherry-Tag Monoclonal Ab at different dilution.
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Product Description

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information

Host SpeciesMouse
Immunogen / Amino acidsRecombinant protein of mCherry tag
IsotypeIgG

Specifications

Storage BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Tested ApplicationWB,IF
Recommended DilutionWB 1:2000 - 1:5000
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesmCherry; mCherry tag; mCherry-tag
SequenceMLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
PurificationAffinity purification
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